Probability of microbiome stripped damselfish’s offspring

by Kelsey Drummond

Submitted : Spring 2021

This experiment was a microbials and probability study. It started out with gathering up a total of 50 three spot damselfish and finding fish with specific genotypes while also having 10 females and 10 males. Following this each fish was placed in their individual tanks and their microbials (microbiome) was stripped off using antibiotics. Then wild caught gobies were used to reintroduce bacteria to the damselfish to give them a “new” microbiome. After this the fish were bred and eggs were laid. This is the first part of the experiment. The second part will consist of testing the offspring gene sequence and compare them to the probability work in this paper. To get an idea of what the outcome should be Punnett squares were used to make a probability guess as to the genotype of the offspring. The Punnett squares showed that a majority of the fish should not have a change in microbials and not show any side effects, but they are possible. The likelihood for all three side effects and a change in microbials had such a low percentage that is very unlikely. The data showed that it is a lot more likely to see one of the side effects and a microbial change than anything else.

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